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Key Features:
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High-Resolution Accurate Mass (HRAM): Combines quadrupole selectivity with TOF resolution for both qualitative and quantitative workflows.
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Ultra-Fast Acquisition Speed: Capable of >100 spectra/sec for compatibility with UHPLC separations.
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High Sensitivity: fg-level detection enables analysis of low-abundance compounds in complex matrices.
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Dynamic Range: >5 orders of magnitude for simultaneous quantitation of trace and major analytes.
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SWATH® Acquisition Technology: Enables comprehensive data-independent acquisition (DIA) for proteomics and metabolomics.
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Powerful Ion Optics: Optimized for high ion transmission and reduced background noise.
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Dual Ion Sources: Supports ESI and APCI for wide application coverage.
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Flexible Acquisition Modes: Information-dependent acquisition (IDA), MS/MSALL, and targeted MS/MS for discovery and verification workflows.
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Robust Hardware Design: Engineered for long-term stability and reproducibility in high-throughput labs.
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Software Ecosystem: SCIEX OS and Analyst® TF software for acquisition, data processing, and regulatory compliance.
Applications:
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Proteomics: Comprehensive protein identification, biomarker discovery, SWATH-based quantitation.
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Metabolomics & Lipidomics: Untargeted and targeted profiling of metabolites and lipids.
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Pharmaceutical Research: Drug metabolism (DMPK), pharmacokinetics (PK/PD), impurity identification, metabolite profiling.
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Clinical & Translational Research: Biomarker discovery, quantitative verification studies.
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Food & Environmental Testing: Screening for contaminants, toxins, pesticides, and emerging pollutants.
Specifications (Table):
| Parameter | Specification |
|---|---|
| System Configuration | AB Sciex TripleTOF 5600+ Mass Spectrometer |
| MS Type | Hybrid Quadrupole-Time-of-Flight (Q-TOF) |
| Mass Range | m/z ~50 – 40,000 (depending on mode) |
| Resolution | >30,000 FWHM |
| Mass Accuracy | <1 ppm with internal calibration |
| Dynamic Range | >5 orders of magnitude |
| Acquisition Speed | >100 spectra/sec |
| Ionization Modes | ESI, APCI |
| Polarity Switching | <50 ms |
| Acquisition Modes | Full Scan MS, IDA, SWATH DIA, MS/MSALL, Product Ion |
| LOD/LOQ Sensitivity | fg–pg level detection (compound dependent) |
| Collision Cell | Q2 linear accelerator collision cell |
| Vacuum System | Turbo molecular pumps with differential pumping |
| Interface | Turbo V™ Source, optional DuoSpray® |
| Software | SCIEX OS, Analyst® TF |
| Compliance | 21 CFR Part 11, GLP/GMP ready |