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Shimadzu Nexera X2 UHPLC System  high-pressure, high-resolution, ultra-fast UHPLC platform for pharmaceuticals, proteomics, food safety, environmental testing, clinical research, and advanced analytical applications.
  • Key Features:

    • The Shimadzu Nexera X2 UHPLC System is a next-generation, high-speed, high-resolution chromatography platform engineered for advanced analytical and bioanalytical applications.

    • With a pressure tolerance up to 130 MPa (18,850 psi), the Nexera X2 supports sub-2 µm and core-shell particle columns, ensuring ultra-high resolution and rapid separations.

    • Offers binary, ternary, and quaternary gradient options, giving labs flexibility for method development and complex solvent systems.

    • Incorporates ultra-fast autosampler with high-precision injections, ultra-low carryover (<0.0015%), and temperature control for sensitive biological samples.

    • Column oven with dual-heating technology (forced air circulation and precise column heating) for temperature stability and reproducibility.

    • Fully compatible with advanced detectors: PDA, fluorescence, RI, ELSD, and seamlessly couples with high-resolution and triple quadrupole mass spectrometers.

    • Controlled through Shimadzu LabSolutions software with full GLP/GMP compliance and 21 CFR Part 11 data integrity support.

    • Modular, scalable design allows custom configuration for analytical, bioanalytical, and MS-coupled workflows.

  • Applications:

    • Pharmaceutical & Biopharmaceutical: Drug discovery, method development, impurity profiling, stability testing, QC in GMP-regulated environments.

    • Proteomics & Metabolomics: High-throughput separation of peptides, proteins, metabolites, amino acids, and complex biomolecules.

    • Food & Nutraceuticals: Analysis of vitamins, preservatives, aflatoxins, natural products, and nutritional profiling.

    • Environmental Testing: Trace-level detection of pesticides, herbicides, and organic pollutants.

    • Clinical & Biomedical Research: Bioanalysis of drugs and metabolites, biomarker assays, pharmacokinetics, hormone testing.

    • Forensic & Toxicology: Screening of drugs of abuse, toxins, and trace contaminants.

    • Industrial & Specialty Chemicals: Polymer characterization, purity testing, and process monitoring.

  • Specifications:

Parameter Specification
System Type Modular UHPLC system
Pressure Rating Up to 130 MPa (18,850 psi)
Flow Rate Range 0.0001–5.0 mL/min (precise flow control)
Pump Options Binary, ternary, and quaternary gradient solvent delivery
Gradient Accuracy ±0.1% or better
Autosampler 0.1–100 µL injection range, cooling/heating capability (4–40 °C), ultra-low carryover <0.0015%
Column Oven Ambient +4 °C to 90 °C, dual heating system, column-switching capability
Detector Options UV-Vis, PDA, fluorescence, RID, ELSD, MS coupling
Software Shimadzu LabSolutions CDS (GLP/GMP and 21 CFR Part 11 compliant)
Carryover <0.0015% (ultra-low)
Sample Throughput Ultra-fast analysis cycles, high sample capacity
Dimensions Modular, stackable design to save bench space


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