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Agilent Revident LC/Q-TOF with 1290 Infinity III UHPLC – high-resolution quadrupole time-of-flight LC-MS system optimized for small molecule screening, pharmaceutical development, metabolomics, food safety, and environmental analysis.

Key Features:

  • Latest-Generation Q-TOF Platform: Designed for high-resolution, accurate-mass (HRAM) analysis with exceptional stability and reproducibility.

  • Integrated 1290 Infinity III UHPLC: Provides ultrahigh chromatographic resolution with precision flow control for robust separations.

  • High Mass Resolution: >60,000 FWHM for confident identification of small molecules in complex samples.

  • Superior Mass Accuracy: <1 ppm with reference mass correction, ensuring precise compound identification.

  • Fast Polarity Switching: <20 ms positive/negative switching for comprehensive small molecule profiling.

  • Flexible Ionization Sources: Compatible with Agilent Jet Stream ESI, APCI, and multimode sources.

  • Multiple Acquisition Modes: Full scan, AutoMS/MS, targeted MS/MS, DIA, and DDA for both discovery and targeted workflows.

  • Dynamic Range & Sensitivity: 5–6 orders of magnitude dynamic range for trace-level detection alongside major components.

  • High-Speed Acquisition: Up to 50 spectra/sec, supporting UHPLC workflows with sharp peak widths.

  • Enhanced Small Molecule Workflows: Optimized for pharmaceuticals, food safety, environmental testing, and metabolomics.

  • Integrated Software: Agilent MassHunter and molecular feature extraction tools for compound identification, library searching, and regulatory compliance.

  • Regulatory Ready: 21 CFR Part 11 support for GLP/GMP environments.

  • Scalable & Modular: Configurable with automation, high-throughput autosamplers, and laboratory informatics.


Applications:

  • Pharmaceutical Development: Small molecule drug screening, metabolite identification, and impurity profiling.

  • Metabolomics & Lipidomics: Untargeted and targeted workflows for biomarker discovery.

  • Food Safety & Quality Control: Detection of pesticides, contaminants, adulterants, and authenticity testing.

  • Environmental Analysis: Monitoring pollutants, pesticides, pharmaceuticals, and emerging contaminants.

  • Forensics & Toxicology: Screening for drugs of abuse, toxins, and forensic unknowns.

  • Clinical & Translational Research: Discovery of metabolic pathways and disease biomarkers.

  • Academic Research: Comprehensive small molecule screening in chemistry and biology labs.


Specifications (Table):

Parameter Specification
LC/MS Platform Agilent 1290 Infinity III UHPLC with Revident LC/Q-TOF
MS System Type Quadrupole Time-of-Flight (Q-TOF)
Mass Range m/z 20 – 20,000
Resolving Power >60,000 FWHM
Mass Accuracy <1 ppm with reference mass correction
Ionization Modes ESI with Jet Stream Technology, APCI, multimode ion sources
Polarity Switching <20 ms
Acquisition Modes Full scan, AutoMS/MS, DIA, DDA, targeted MS/MS
Acquisition Rate Up to 50 spectra/sec
Dynamic Range 5–6 orders of magnitude
Fragmentation Collision-induced dissociation (CID)
Vacuum System Turbo molecular pumps with differential pumping
Interface Orthogonal spray ion source with ion optics
Autosampler InfinityLab series autosamplers
Software Agilent MassHunter, molecular feature extraction, compound databases
Compliance 21 CFR Part 11 support, GLP/GMP ready


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