Protein Purification (FPLC)

Fast Protein Liquid Chromatography (FPLC) is a liquid chromatography technique designed for the separation and purification of proteins, peptides, and biomolecules. Unlike conventional HPLC, FPLC systems operate at lower pressures and use buffers that maintain protein stability during purification.

FPLC systems are widely used in biopharmaceutical research, drug development, and academic laboratories for:

• Protein purification from complex mixtures

• Biomolecule fractionation (enzymes, antibodies, peptides)

• Sample preparation prior to structural or functional analysis

Popular platforms include Cytiva ÄKTA systems (ÄKTA pure, ÄKTA avant, ÄKTA start), known for reliability, gradient precision, and scalability from research labs to production.