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The Cytiva ÄKTA Pure 25 M is a high-performance, modular FPLC system designed for automated protein purification in research, process development, and quality control laboratories. With advanced UV detection, precision pumping, and automated fraction collection, it ensures reproducible, efficient, and scalable purification workflows.
Key Features:
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Modular System: Easily customizable with F9 Fraction Collector, S9 Sample Pump, and optional modules for UV, conductivity, and mixers.
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High-Performance Pumping: Dual-piston pump allows up to 25 mL/min flow and 20 MPa pressure for reproducible separations.
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Advanced Detection: UV detector for accurate analyte monitoring; supports single, dual, or multi-wavelength options.
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Automated Fraction Collection: F9 Fraction Collector simplifies workflow and enhances reproducibility.
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User-Friendly Software: UNICORN™ software for method development, system control, and data analysis.
Applications:
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Recombinant protein purification
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Antibody and peptide purification
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Nucleic acid isolation (plasmid DNA, RNA)
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Process development and optimization
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Analytical studies of biomolecular interactions
Specifications:
| Feature | Details |
|---|---|
| Flow Rate | 0.001 – 25 mL/min (up to 50 mL/min during column packing) |
| Pressure Range | 0 – 20 MPa |
| UV Detection | Single-wavelength (280 nm), optional dual/multi-wavelength |
| Mixer Volume | 0.6 mL, 1.4 mL, 5 mL |
| Dimensions (W × H × D) | 535 × 630 × 470 mm |
| Weight | Up to 53 kg (excluding computer, pump, fraction collector) |
| Power Supply | 100–240 V, 50–60 Hz |
| Power Consumption | 300 VA (typical), 25 VA (power-save) |
| Enclosure Class | IP 21 |
Included Components:
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Dual-piston S9 Sample Pump
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F9 Fraction Collector
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Gradient Mixer (1.4 mL standard)
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Injection Valve (V9-Inj)
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UV Detector (U9-M or U9-L depending on configuration)
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Conductivity Monitor (0.01 – 999.99 mS/cm)