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Cytiva ÄKTA Pure 150 K FPLC System with Fraction Collector and UV Detector, high-capacity fast protein liquid chromatography platform for large-scale protein purification, biopharmaceutical research, process development, and industrial biotechnology applications.

The Cytiva ÄKTA Pure 150 K is a high-capacity, modular FPLC system designed for efficient and scalable protein purification. Engineered for both research and process development, it offers flexibility, precision, and automation to meet diverse purification needs.


Key Features:

  • High Flow Rate:
    Capable of handling flow rates up to 150 mL/min, suitable for large-scale preparative purification.

  • Modular Design:
    Offers over 20 hardware options, including F9 Fraction Collector and S9 Sample Pump, allowing customization to specific application requirements.

  • Advanced Detection:
    Equipped with a UV detector for precise monitoring of analytes, enhancing the accuracy of purification processes.

  • Automated Fraction Collection:
    The F9 Fraction Collector automates the collection of elution fractions, streamlining workflows and improving reproducibility.

  • Intuitive Software Control:
    Operated via UNICORN™ software, facilitating method development, system control, and data analysis.


Applications:

  • Protein Purification:
    Ideal for purifying recombinant proteins, antibodies, and other biomolecules.

  • Nucleic Acid Purification:
    Suitable for isolating plasmid DNA, RNA, and other nucleic acids.

  • Process Development:
    Assists in the development and optimization of purification workflows.

  • Analytical Applications:
    Supports studies of biomolecular interactions and purity assessments.


Specifications:

Feature Details
Flow Rate 0.01 to 150 mL/min (up to 300 mL/min during column packing)
Pressure Range 0 to 5 MPa (50 bar)
UV Detection Single-wavelength (280 nm), options for dual/multi-wavelength
Mixer Volume 1.4 mL (mounted on system), 5 mL, 15 mL
Dimensions (W × H × D) 535 × 630 × 470 mm
Weight Up to 53 kg (excluding computer, sample pump, fraction collector)
Power Supply 100–240 V, ~50–60 Hz
Power Consumption 300 VA (typical), 25 VA (power-save)
Enclosure Class IP 21

Included Components:

  • Dual-piston S9 Sample Pump

  • F9 Fraction Collector

  • Gradient Mixer (1.4 mL standard)

  • Injection Valve (V9-Inj)

  • UV Detector (U9-M or U9-L depending on configuration)

  • Conductivity Monitor (0.01 – 999.99 mS/cm)

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