The Cytiva ÄKTA Avant 150 is a high-capacity, modular FPLC system designed for pilot-scale protein purification and process development. Built to bridge lab-scale optimization with large-scale production, the Avant 150 delivers scalability, precision, and automation through the intuitive UNICORN software.

Key Features

• Generous Flow Range: Operates from 0.01 mL/min up to 150 mL/min for purification; supports column packing at up to 300 mL/min.

• Robust Pressure Operation: Rated for pressures up to 5 MPa (50 bar / 725 psi).

• Full-Spectrum UV Detection: Monitors 190–700 nm at up to three wavelengths simultaneously.

• Automated Buffer & Sample Handling: Supports up to 18 buffer inlets (expandable to 46 with BufferPro) and automated injection of up to 7 samples.

• Wide Chromatography Mode Support: Compatible with affinity, ion exchange, size exclusion, and hydrophobic interaction chromatography.

• Pilot-Scale Column Flexibility: Handles columns up to 75 mm diameter and 40 cm height.

• UNICORN Control Software: Enables method design, automation workflows, and gradient control.

• Integrated Fraction Collector: Real-time monitoring with UV, conductivity, and optional pH tracking.

Applications

• Pilot-scale recombinant protein and antibody purification

• Process development and optimization workflows

• Column packing and resin evaluation for scale-up

• Nucleic acid purification (DNA, RNA, plasmids)

• High-throughput method development and automation

Specifications