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The Cytiva ÄKTA Avant 25 is a leading-edge, preparative FPLC system tailored for swift and secure development of scalable protein purification processes. Whether you're conducting resin screening, method optimization, or process scale-up, Avant 25 delivers precise, reliable results powered by intuitive UNICORN software.
🔑 Key Features
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Preparative Process Focus: Designed for chromatography resin screening, method development, and seamless process scale-up workflows.
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Wide Detection Range: UV detection covers 190–700 nm, allowing multi-wavelength monitoring for diverse biomolecule types.
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Flexible Flow Control: Operates from 0.001 to 25 mL/min during purification, and up to 50 mL/min during column packing.
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Robust Pressure Handling: Maximum operating pressure reaches 20 MPa (200 bar), supporting high-backpressure applications.
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Advanced Automation Tools: Features integrated UNICORN software with Design of Experiments (DoE), BufferPro online buffer preparation, and cooling fraction collection for enhanced process control.
🎯 Applications
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Medium-scale recombinant protein and antibody purification
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Resin screening and method optimization during process development
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Scale-up validation from laboratory to pilot-scale purification workflows
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Analytical method design using DoE and automated gradient profiling
📊 Specifications
| Parameter | Details |
|---|---|
| Flow Rate | 0.001–25 mL/min (purification); up to 50 mL/min (column packing) |
| Pressure Range | 0–20 MPa (200 bar / 2900 psi) |
| UV Detection | 190–700 nm, up to 3 wavelengths simultaneously |
| Dimensions (W × D × H) | 860 × 710 × 660 mm |
| Weight | Approx. 116 kg (excluding control PC) |
| Power Supply | 100–240 V, 50–60 Hz; ~800 VA consumption |
| Control Software | UNICORN (with DoE, BufferPro, method automation) |
| Fraction Collector | Built-in cooled collector for safe, high-throughput fractionation |