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SCIEX QTRAP 6500+ LC-MS/MS with Agilent 1290 Infinity II UHPLC  ultra-sensitive triple quadrupole linear ion trap system for pharmaceutical, clinical, food safety, and environmental targeted analysis.

Key Features:

  • Hybrid QTRAP Technology: Combines triple quadrupole MRM quantitation with linear ion trap functionality for enhanced qualitative analysis.

  • Unmatched Sensitivity: Achieves fg-level detection with exceptional reproducibility across complex matrices.

  • Fast Polarity Switching: <5 ms polarity switching for seamless detection of positive and negative ions in a single run.

  • High-Speed MRM Transitions: Up to 30,000 MRM transitions/sec for robust quantitation in high-throughput assays.

  • Scheduled MRM™ Pro & MRM³: Optimized dwell times and enhanced selectivity for trace analyte detection.

  • SelexION®+ Differential Mobility Separation (optional): Adds another dimension of separation for complex samples.

  • Stable Ion Drive™ Turbo V Source: Improved ionization efficiency with extended robustness and reduced maintenance.

  • Dynamic Range: Wide quantitative linear dynamic range of >6 orders of magnitude.

  • Agilent 1290 Infinity II UHPLC: Delivers ultra-high chromatographic resolution with fast gradients, sub-2 μm column compatibility, and precise sample injection.

  • Software Ecosystem: SCIEX OS for acquisition, processing, regulatory compliance, and automation.

  • Regulatory Compliance: Full support for 21 CFR Part 11, GLP, and GMP workflows.


Applications:

  • Pharmaceutical & Biopharma: Pharmacokinetics (PK), drug metabolism studies, impurity profiling, biomarker quantitation.

  • Clinical Research & Toxicology: Quantification of therapeutic drugs, metabolites, and forensic toxicology.

  • Food & Environmental Testing: Detection of pesticide residues, veterinary drugs, mycotoxins, contaminants, and pollutants.

  • Proteomics & Metabolomics: Targeted peptide quantitation, small molecule analysis, biomarker verification.

  • Biologics Development: Quantification of peptides, proteins, and oligonucleotides in regulated workflows.


Specifications (Table):

Parameter Specification
System Configuration SCIEX QTRAP 6500+ Triple Quad/Linear Ion Trap + Agilent 1290 Infinity II UHPLC
MS Type Hybrid Triple Quadrupole / Linear Ion Trap
Mass Range m/z ~5 – 2000
Resolution Unit resolution (0.7 Da FWHM)
Dynamic Range >6 orders of magnitude
Ionization Modes ESI, APCI (Turbo V™ Ion Source)
Polarity Switching <5 ms
Acquisition Modes MRM, Scheduled MRM™ Pro, MRM³, Product Ion, Enhanced MS (EMS), Enhanced Product Ion (EPI)
MRM Transition Speed Up to 30,000 transitions/sec
LOD/LOQ Sensitivity fg-level detection (compound dependent)
Collision Cell Linear accelerator collision cell with QJet® ion guide
Vacuum System Turbo molecular pump with differential pumping
Front-End UHPLC Agilent 1290 Infinity II (binary pump, autosampler, thermostatted column compartment, diode array detector)
Autosampler Precision ≤0.25% RSD
Flow Range 0.001 – 5 mL/min
Gradient Precision ≤0.05% RSD
Interface Turbo V™ Source with optional SelexION®+ differential mobility
Software SCIEX OS (data acquisition, processing, compliance-ready reporting)
Compliance 21 CFR Part 11, GLP/GMP compliant


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