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Key Features:
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Hybrid QTRAP Technology: Combines triple quadrupole MRM quantitation with linear ion trap functionality for enhanced qualitative analysis.
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Unmatched Sensitivity: Achieves fg-level detection with exceptional reproducibility across complex matrices.
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Fast Polarity Switching: <5 ms polarity switching for seamless detection of positive and negative ions in a single run.
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High-Speed MRM Transitions: Up to 30,000 MRM transitions/sec for robust quantitation in high-throughput assays.
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Scheduled MRM™ Pro & MRM³: Optimized dwell times and enhanced selectivity for trace analyte detection.
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SelexION®+ Differential Mobility Separation (optional): Adds another dimension of separation for complex samples.
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Stable Ion Drive™ Turbo V Source: Improved ionization efficiency with extended robustness and reduced maintenance.
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Dynamic Range: Wide quantitative linear dynamic range of >6 orders of magnitude.
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Agilent 1290 Infinity II UHPLC: Delivers ultra-high chromatographic resolution with fast gradients, sub-2 μm column compatibility, and precise sample injection.
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Software Ecosystem: SCIEX OS for acquisition, processing, regulatory compliance, and automation.
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Regulatory Compliance: Full support for 21 CFR Part 11, GLP, and GMP workflows.
Applications:
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Pharmaceutical & Biopharma: Pharmacokinetics (PK), drug metabolism studies, impurity profiling, biomarker quantitation.
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Clinical Research & Toxicology: Quantification of therapeutic drugs, metabolites, and forensic toxicology.
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Food & Environmental Testing: Detection of pesticide residues, veterinary drugs, mycotoxins, contaminants, and pollutants.
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Proteomics & Metabolomics: Targeted peptide quantitation, small molecule analysis, biomarker verification.
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Biologics Development: Quantification of peptides, proteins, and oligonucleotides in regulated workflows.
Specifications (Table):
| Parameter | Specification |
|---|---|
| System Configuration | SCIEX QTRAP 6500+ Triple Quad/Linear Ion Trap + Agilent 1290 Infinity II UHPLC |
| MS Type | Hybrid Triple Quadrupole / Linear Ion Trap |
| Mass Range | m/z ~5 – 2000 |
| Resolution | Unit resolution (0.7 Da FWHM) |
| Dynamic Range | >6 orders of magnitude |
| Ionization Modes | ESI, APCI (Turbo V™ Ion Source) |
| Polarity Switching | <5 ms |
| Acquisition Modes | MRM, Scheduled MRM™ Pro, MRM³, Product Ion, Enhanced MS (EMS), Enhanced Product Ion (EPI) |
| MRM Transition Speed | Up to 30,000 transitions/sec |
| LOD/LOQ Sensitivity | fg-level detection (compound dependent) |
| Collision Cell | Linear accelerator collision cell with QJet® ion guide |
| Vacuum System | Turbo molecular pump with differential pumping |
| Front-End UHPLC | Agilent 1290 Infinity II (binary pump, autosampler, thermostatted column compartment, diode array detector) |
| Autosampler Precision | ≤0.25% RSD |
| Flow Range | 0.001 – 5 mL/min |
| Gradient Precision | ≤0.05% RSD |
| Interface | Turbo V™ Source with optional SelexION®+ differential mobility |
| Software | SCIEX OS (data acquisition, processing, compliance-ready reporting) |
| Compliance | 21 CFR Part 11, GLP/GMP compliant |