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Thermo Scientific Q Exactive HF-X Orbitrap Mass Spectrometer with Vanquish UHPLC High-speed high-resolution LC-MS/MS system for advanced proteomics and metabolomics research
  • Thermo Scientific Q Exactive HF-X Orbitrap Mass Spectrometer with Vanquish UHPLC High-speed high-resolution LC-MS/MS system for advanced proteomics and metabolomics research
  • Thermo Scientific Q Exactive HF-X Orbitrap Mass Spectrometer with Vanquish UHPLC High-speed high-resolution LC-MS/MS system for advanced proteomics and metabolomics research
Thermo Scientific Q Exactive™ HF-X Orbitrap LC-MS/MS with Vanquish UHPLC Next-Generation LC-MS/MS for Deep Proteome Analysis
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  • Key Features:
    • Highest-performance model in the Q Exactive series, optimized for deep proteome coverage and high-throughput omics
    • Resolution up to 240,000 FWHM at m/z 200 with sub-ppm mass accuracy
    • Ultra-fast sequencing speeds with improved parallelization and ion optics, enabling analysis of >6,000 proteins in a single run
    • Significantly enhanced sensitivity and lower limits of detection compared to Q Exactive HF
    • Ideal for DIA and PRM workflows, supporting quantitative reproducibility at scale
    • Integrated with Thermo Vanquish UHPLC for robust, high-throughput, and reproducible chromatographic separations
    • Optimized for large-scale proteomics, single-cell proteomics, and high-complexity samples
    • Comprehensive software ecosystem: Xcalibur™, Proteome Discoverer™, TraceFinder™
    • Designed for demanding environments, offering reliability and reproducibility for continuous operation

  • Applications:
    • Proteomics: Deep protein identification, quantitation, PTM characterization, and single-cell proteomics
    • Metabolomics & Lipidomics: High-resolution profiling of small molecules and complex mixtures
    • Biopharma: Advanced peptide mapping, glycan analysis, and intact protein characterization
    • Clinical & Translational Research: Quantitative biomarker discovery and validation studies
    • Large-Scale Systems Biology: High-throughput omics workflows requiring speed and sensitivity

  • Specifications:

    Specification Details
    MS Platform Thermo Scientific Q Exactive HF-X Orbitrap
    MS System Type Hybrid Quadrupole-Orbitrap LC-MS/MS
    Coupled System Thermo Scientific Vanquish UHPLC
    Ionization Source Electrospray Ionization (ESI), nano-ESI
    Mass Analyzers Quadrupole + Orbitrap
    Mass Resolution Up to 240,000 FWHM at m/z 200
    Mass Accuracy <1 ppm with external calibration
    Fragmentation Methods Higher-energy Collisional Dissociation (HCD)
    Polarity Modes Positive and negative switching
    Dynamic Range >6 orders of magnitude
    Sensitivity Sub-femtomole to attomole detection levels
    Acquisition Modes Full MS, MS/MS, DIA, PRM
    Software Thermo Xcalibur™, Proteome Discoverer™, TraceFinder™
    Compliance 21 CFR Part 11 ready

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