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AB Sciex 4500 Triple Quadrupole LC-MS/MS with Agilent 1290 Infinity UHPLC – ultra-sensitive, high-throughput quantitative LC-MS/MS system for pharmaceutical, food safety, environmental, and clinical applications.

Key Features:

  • Integrated LC-MS Solution: Combines the robust quantitation power of the AB Sciex 4500 Triple Quad with the precision and throughput of the Agilent 1290 Infinity UHPLC.

  • Superior Chromatographic Resolution: Ultra-high-pressure UHPLC delivers sharper peaks and faster separations.

  • High-Sensitivity MRM Quantitation: Capable of detecting trace analytes with reproducible quantitative accuracy.

  • Fast Polarity Switching: <20 ms for workflows requiring both positive and negative ion transitions.

  • Dynamic MRM (dMRM): Automatically optimizes dwell and cycle times for large multi-analyte panels.

  • Versatile Ion Sources: Compatible with Turbo V™ ion source, DuoSpray®, and APCI.

  • Advanced Collision Cell: LINAC® collision cell technology for reduced crosstalk and enhanced signal.

  • Wide Dynamic Range: Accurately quantifies both trace-level and high-abundance compounds.

  • Regulatory Compliance: Supports 21 CFR Part 11 for GLP/GMP-regulated environments.

  • Optimized for Routine High-Throughput Labs: Rugged design and low maintenance requirements for continuous operation.


Applications:

  • Pharmaceutical R&D and QC: Drug metabolism, pharmacokinetics, bioequivalence studies, impurity analysis.

  • Food & Beverage Safety: Monitoring pesticides, veterinary residues, mycotoxins, and other contaminants.

  • Environmental Testing: Quantification of pollutants, trace organics, and emerging contaminants.

  • Clinical & Forensic Analysis: Therapeutic drug monitoring, toxicology, and forensic screening.

  • Biological Research: Targeted proteomics and metabolomics requiring fast UHPLC separation and precise MS quantitation.


Specifications (Table):

Parameter Specification
MS Type Triple Quadrupole LC-MS/MS
Mass Range m/z 5 – 2,000
Mass Resolution Unit resolution (0.7 Da FWHM)
Polarity Switching <20 ms
Dynamic Range 5–6 orders of magnitude
Scan Speed Up to 5,000 Da/sec
Acquisition Modes MRM, Product Ion, Precursor Ion, Neutral Loss, Full Scan
Ionization Sources Turbo V™, DuoSpray®, APCI
UHPLC System Agilent 1290 Infinity UHPLC
UHPLC Pressure Up to 1,200 bar
UHPLC Flow Rate Range 0.001 – 5.0 mL/min
UHPLC Column Heating 10 – 100 °C
Autosampler Integrated Agilent 1290 Infinity with low carryover
Collision Cell LINAC® collision cell technology
Vacuum System Dual turbomolecular pumps
Software Analyst® Software (MS), Agilent OpenLab CDS or ChemStation (UHPLC)
Compliance 21 CFR Part 11, GLP/GMP ready


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