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Key Features:
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Hybrid QTRAP Architecture: Combines triple quadrupole MRM quantitation with linear ion trap scanning, enabling both targeted assays and qualitative workflows.
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High Sensitivity: Significantly improved signal-to-noise ratio over the 4500 QTRAP, designed for fg–pg level detection in complex matrices.
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High-Speed Quantitation: Handles up to 1000 MRM transitions/sec with optimized dwell times, suited for UHPLC separations.
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Enhanced Product Ion (EPI) and MS³: Enables structural elucidation, impurity profiling, and metabolite identification.
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Polarity Switching: Ultra-fast (<50 ms) switching between positive and negative ionization modes.
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Turbo V™ Source: Robust and flexible with interchangeable ESI and APCI probes, supporting a wide compound range.
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Dynamic Range: Broad quantitation range (5–6 orders of magnitude) for trace-level and high-concentration compounds.
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Compact, Robust Design: Engineered for demanding analytical labs with high-throughput workflows.
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Regulatory Compliance: Full support for FDA 21 CFR Part 11, GLP/GMP readiness, and validated workflows.
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Software Ecosystem: SCIEX Analyst®, MultiQuant™, and supporting bioanalytical data solutions.
Applications:
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Pharmaceutical & Biopharma: Quantitative bioanalysis, DMPK studies, pharmacokinetics, metabolite ID, impurity testing.
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Food Safety & Agriculture: Detection of pesticide residues, mycotoxins, veterinary drugs, and contaminants in food products.
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Environmental Testing: Monitoring pharmaceuticals, endocrine disruptors, and trace organic pollutants in environmental matrices.
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Clinical & Forensic Toxicology: Drug screening, toxicology testing, clinical biomarker quantitation.
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Metabolomics & Lipidomics: Targeted metabolite profiling with confirmatory structural analysis.
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Academic & Translational Research: Supports hybrid workflows combining MRM quantitation with qualitative confirmation.
Specifications (Table):
Parameter | Specification |
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System Configuration | AB SCIEX 5500 QTRAP LC-MS/MS |
MS Type | Hybrid Triple Quadrupole – Linear Ion Trap (QTRAP) |
Mass Range | m/z 5 – 2000 |
Resolution | Unit resolution (0.7 Da FWHM) |
Dynamic Range | ~5–6 orders of magnitude |
Ionization Modes | Turbo V™ Source (ESI, APCI) |
Polarity Switching | <50 ms |
Acquisition Modes | MRM, SIM, EPI, MS³, Precursor Ion Scan, Neutral Loss Scan |
MRM Transition Speed | Up to 1000 transitions/sec |
Sensitivity (LOQ) | fg–pg level depending on analyte |
Collision Cell | Q2 linear collision cell |
Vacuum System | Turbo molecular pumping system |
Front-End Compatibility | Works with most UHPLC/HPLC systems (Agilent, Shimadzu, Waters, Thermo) |
Autosampler | UHPLC/HPLC autosamplers compatible |
Interface | Turbo V ion source with orthogonal spray |
Software | SCIEX Analyst®, MultiQuant™ |
Compliance | 21 CFR Part 11, GLP/GMP support |