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Key Features:
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Modular Agilent 1100 HPLC system configured with quaternary pump, autosampler, column oven, variable wavelength detector (VWD), and refractive index detector (RID).
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Quaternary pump allows mixing of up to four solvents with isocratic or gradient elution, supporting flexible method development.
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High-pressure mixing with vacuum degassing ensures stable baselines and reproducible chromatography.
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Autosampler with precise programmable injection volumes and high sample capacity for unattended operation.
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Column oven (thermostatted compartment) provides precise temperature control for reproducible retention times and improved chromatographic resolution.
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Variable Wavelength Detector (VWD):
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Selectable wavelength monitoring in the 190–600 nm range.
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High sensitivity for analytes with UV absorbance, ideal for pharmaceuticals, organics, and biomolecules.
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Refractive Index Detector (RID):
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Universal detector for compounds lacking strong chromophores (e.g., sugars, polymers, alcohols, organic acids).
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High-stability optical bench for accurate and reproducible measurements.
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Supports a wide range of chromatographic modes: reversed-phase, normal-phase, ion-exchange, size-exclusion, and specialty columns.
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Fully integrated with Agilent ChemStation software for system control, data processing, quantitation, reporting, and 21 CFR Part 11 compliance.
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Reliable, rugged, and widely adopted for both research and regulated environments.
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Applications:
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Pharmaceutical & Biotech: Assay and impurity testing, drug stability studies, active pharmaceutical ingredient (API) analysis, formulation testing.
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Food & Beverage: Sugars, organic acids, alcohols, additives, preservatives, sweeteners, and quality control testing.
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Environmental Analysis: Pesticides, herbicides, and water-soluble pollutants not strongly UV-absorbing.
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Chemical & Industrial: QA/QC of raw materials, specialty chemicals, surfactants, and polymers.
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Clinical & Biomedical Research: Analysis of carbohydrates, nucleotides, and compounds with weak UV absorbance.
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Academic & R&D Laboratories: Teaching, method development, and general-purpose chromatography research.
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Specifications:
Parameter | Specification |
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System Type | High-Performance Liquid Chromatography (HPLC) |
Pump | Quaternary gradient pump, 4-solvent mixing |
Flow Range | Typically 0.001–10 mL/min |
Pressure Range | Up to 400 bar (quaternary pump) |
Degasser | Integrated vacuum degassing unit |
Autosampler | Injection range: 0.1–100 µL programmable, high sample tray capacity |
Column Oven | Thermostatted, 10–80°C (extendable with options) |
Variable Wavelength Detector (VWD) | Wavelength range: 190–600 nm, selectable single wavelength monitoring |
Refractive Index Detector (RID) | Universal detection, high-stability optics, suitable for non-UV analytes |
Software | Agilent ChemStation, OpenLab CDS |
Compliance | GLP/GMP, 21 CFR Part 11 |