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Bruker timsTOF Pro with Evosep One LC  High-throughput 4D proteomics LC-MS/MS system with TIMS-PASEF technology for deep proteome analysis, biomarker discovery, and clinical research applications.
  • Key Features:

    • Trapped Ion Mobility Spectrometry (TIMS) combined with Parallel Accumulation–Serial Fragmentation (PASEF) for ultra-high sensitivity and speed.

    • Integrated with Evosep One LC for robust, high-throughput sample loading and separation.

    • 4D proteomics workflow enables separation by retention time, mass-to-charge ratio, ion mobility, and intensity.

    • Exceptional quantitative reproducibility for large cohort proteomic studies.

    • High duty cycle with minimal sample carryover, ideal for clinical proteomics.

    • Optimized for deep proteome coverage with improved peptide and protein identifications.

    • Compact, robust design suitable for core labs, pharma, and research centers.

  • Applications:

    • Large-scale quantitative proteomics and biomarker discovery.

    • Clinical research and translational medicine studies.

    • Pharmaceutical drug development and biomarker validation.

    • Systems biology and pathway analysis.

    • Deep tissue proteomics, cancer research, and personalized medicine.

    • High-throughput proteomic workflows with low sample consumption.

  • Specifications:

Parameter Specification
LC Platform Evosep One LC with predefined high-throughput gradient methods
MS System Type Quadrupole Time-of-Flight (QTOF) with Trapped Ion Mobility Spectrometry (TIMS)
Ionization Modes ESI (Electrospray Ionization), positive/negative mode
Autosampler Integrated with Evosep One LC (nanoflow, high-throughput sample loader)
Acquisition Modes PASEF, DDA (Data-Dependent Acquisition), DIA (Data-Independent Acquisition)
Dynamic Range >5 orders of magnitude
Mass Accuracy <1 ppm (internal calibration)
Resolving Power >60,000 FWHM
Vacuum System Multi-stage turbo pumps optimized for QTOF and TIMS stability
Interface CaptiveSpray nanoBooster source (nanospray ionization)
Software Bruker Compass, DataAnalysis, MaxQuant, Spectronaut compatibility
Compliance 21 CFR Part 11 ready


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