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SCIEX TripleTOF 5600+ LC-MS/MS with Eksigent nanoLC 400  high-resolution QTOF mass spectrometry system for proteomics, metabolomics, pharmaceutical discovery, and translational research applications.

Key Features:

  • Hybrid Q-TOF Design: Quadrupole mass filter coupled with TOF analyzer delivers both selectivity and high-resolution accurate mass detection.

  • High-Resolution Accurate Mass (HRAM): Achieves >30,000 FWHM resolution with sub-ppm mass accuracy for reliable identification.

  • Ultra-Fast Acquisition: >100 spectra/sec, compatible with fast UHPLC and nanoLC separations.

  • SWATH® Acquisition: Data-independent acquisition for reproducible, comprehensive proteomics and metabolomics analysis.

  • Eksigent nanoLC 400 Front-End: Nano-flow LC system optimized for proteomics, delivering high chromatographic resolution with minimal sample consumption.

  • Dynamic Range: >5 orders of magnitude for simultaneous detection of low- and high-abundance analytes.

  • Versatile Ion Sources: Turbo V™ or DuoSpray® ion sources support ESI and APCI for wide compound coverage.

  • Advanced Scan Modes: IDA (Information Dependent Acquisition), MS/MSALL, Product Ion scans for both discovery and targeted analysis.

  • Robust System: Designed for long-term stability, reproducibility, and high-throughput workflows.

  • Software Ecosystem: SCIEX OS and Analyst® TF software for acquisition, processing, and regulatory compliance.


Applications:

  • Proteomics: Deep protein identification, biomarker discovery, SWATH-based quantitative proteomics.

  • Metabolomics & Lipidomics: Untargeted and targeted metabolite and lipid profiling.

  • Pharmaceutical Research: Drug metabolism (DMPK), pharmacokinetics, impurity identification, compound verification.

  • Clinical & Translational Research: Biomarker discovery and verification, disease pathway analysis.

  • Food & Environmental Testing: Screening for contaminants, pesticides, toxins, and pollutants.


Specifications (Table):

Parameter Specification
System Configuration SCIEX TripleTOF 5600+ Hybrid Q-TOF + Eksigent nanoLC 400
MS Type Hybrid Quadrupole-Time-of-Flight (Q-TOF)
Mass Range m/z ~50 – 40,000
Resolution >30,000 FWHM
Mass Accuracy <1 ppm with internal calibration
Dynamic Range >5 orders of magnitude
Acquisition Speed >100 spectra/sec
Ionization Modes ESI, APCI
Polarity Switching <50 ms
Acquisition Modes Full Scan MS, SWATH DIA, IDA, MS/MSALL, Product Ion
Sensitivity fg–pg range (compound dependent)
Collision Cell Q2 linear accelerator collision cell
Vacuum System Differentially pumped turbo molecular system
Front-End LC Eksigent nanoLC 400 (nano-flow chromatography, optimized for proteomics)
Flow Range Nano-flow (nL/min – μL/min range)
Autosampler Precision Optimized for low-volume proteomics samples
Interface Turbo V™ Source, optional DuoSpray®
Software SCIEX OS, Analyst® TF
Compliance 21 CFR Part 11, GLP/GMP ready


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