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Key Features:
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Hybrid Q-TOF Design: Quadrupole mass filter coupled with TOF analyzer delivers both selectivity and high-resolution accurate mass detection.
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High-Resolution Accurate Mass (HRAM): Achieves >30,000 FWHM resolution with sub-ppm mass accuracy for reliable identification.
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Ultra-Fast Acquisition: >100 spectra/sec, compatible with fast UHPLC and nanoLC separations.
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SWATH® Acquisition: Data-independent acquisition for reproducible, comprehensive proteomics and metabolomics analysis.
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Eksigent nanoLC 400 Front-End: Nano-flow LC system optimized for proteomics, delivering high chromatographic resolution with minimal sample consumption.
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Dynamic Range: >5 orders of magnitude for simultaneous detection of low- and high-abundance analytes.
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Versatile Ion Sources: Turbo V™ or DuoSpray® ion sources support ESI and APCI for wide compound coverage.
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Advanced Scan Modes: IDA (Information Dependent Acquisition), MS/MSALL, Product Ion scans for both discovery and targeted analysis.
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Robust System: Designed for long-term stability, reproducibility, and high-throughput workflows.
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Software Ecosystem: SCIEX OS and Analyst® TF software for acquisition, processing, and regulatory compliance.
Applications:
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Proteomics: Deep protein identification, biomarker discovery, SWATH-based quantitative proteomics.
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Metabolomics & Lipidomics: Untargeted and targeted metabolite and lipid profiling.
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Pharmaceutical Research: Drug metabolism (DMPK), pharmacokinetics, impurity identification, compound verification.
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Clinical & Translational Research: Biomarker discovery and verification, disease pathway analysis.
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Food & Environmental Testing: Screening for contaminants, pesticides, toxins, and pollutants.
Specifications (Table):
| Parameter | Specification |
|---|---|
| System Configuration | SCIEX TripleTOF 5600+ Hybrid Q-TOF + Eksigent nanoLC 400 |
| MS Type | Hybrid Quadrupole-Time-of-Flight (Q-TOF) |
| Mass Range | m/z ~50 – 40,000 |
| Resolution | >30,000 FWHM |
| Mass Accuracy | <1 ppm with internal calibration |
| Dynamic Range | >5 orders of magnitude |
| Acquisition Speed | >100 spectra/sec |
| Ionization Modes | ESI, APCI |
| Polarity Switching | <50 ms |
| Acquisition Modes | Full Scan MS, SWATH DIA, IDA, MS/MSALL, Product Ion |
| Sensitivity | fg–pg range (compound dependent) |
| Collision Cell | Q2 linear accelerator collision cell |
| Vacuum System | Differentially pumped turbo molecular system |
| Front-End LC | Eksigent nanoLC 400 (nano-flow chromatography, optimized for proteomics) |
| Flow Range | Nano-flow (nL/min – μL/min range) |
| Autosampler Precision | Optimized for low-volume proteomics samples |
| Interface | Turbo V™ Source, optional DuoSpray® |
| Software | SCIEX OS, Analyst® TF |
| Compliance | 21 CFR Part 11, GLP/GMP ready |