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Shimadzu Prominence HPLC System with RF-20A Fluorescence Detector  high-sensitivity analytical HPLC platform for pharmaceuticals, biomolecules, environmental testing, food safety, and clinical research applications.
  • Key Features:

    • The Shimadzu Prominence HPLC System is a modular high-performance liquid chromatography platform, widely used for analytical and quality control laboratories.

    • Equipped with the RF-20A Fluorescence Detector, offering ultra-high sensitivity and selectivity for compounds that naturally fluoresce or are derivatized with fluorescent tags.

    • Detects analytes at concentrations down to sub-picogram levels, making it ideal for trace analysis.

    • Wavelength range of 200–650 nm (excitation) and 250–750 nm (emission) with high signal-to-noise ratio and rapid response.

    • Capable of 3D fluorescence scanning, allowing determination of excitation/emission spectra and optimization of analytical conditions.

    • Modular design allows integration with pumps, autosamplers, column ovens, and additional detectors (UV, PDA, RID) in the Prominence series.

    • Controlled by Shimadzu LabSolutions software, with full regulatory compliance support for GLP/GMP and 21 CFR Part 11 environments.

  • Applications:

    • Pharmaceutical & Biopharmaceuticals: Quantification of drugs and metabolites, impurity profiling, pharmacokinetic studies.

    • Biochemistry & Life Sciences: Amino acid analysis, protein and peptide detection, nucleic acid derivatives, enzyme assays.

    • Environmental Analysis: Trace detection of PAHs (polycyclic aromatic hydrocarbons), pesticides, and contaminants in water and soil.

    • Food & Nutraceuticals: Vitamin assays, natural product analysis, detection of aflatoxins, food additives, and fluorescent compounds.

    • Clinical Research: Hormone analysis, biomarker studies, clinical pharmacology.

    • Forensic & Toxicology: Screening of fluorescent drug metabolites and toxins.

  • Specifications:

Parameter Specification
System Type Modular HPLC system with fluorescence detection
Detector Model RF-20A Fluorescence Detector
Excitation Wavelength Range 200–650 nm
Emission Wavelength Range 250–750 nm
Sensitivity Sub-picogram-level detection
Features 3D fluorescence scanning, programmable wavelength switching, high S/N ratio
Pump Options Isocratic, binary, or quaternary solvent delivery (flow range 0.001–10 mL/min)
Autosampler High-precision injection, low carryover, variable volume
Column Oven Temperature range: ambient +4 °C to 85 °C
Software Shimadzu LabSolutions CDS
Compliance GLP/GMP, 21 CFR Part 11 compliant


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